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首页> 外文OA文献 >The 5' coding region of Paramecium surface antigen genes controls mutually exclusive transcription.
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The 5' coding region of Paramecium surface antigen genes controls mutually exclusive transcription.

机译：草履虫表面抗原基因的5'编码区控制相互排斥的转录。

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摘要

Paramecium tetraurelia stock 51 can express at least 11 different types of surface antigens, yet only a single type is expressed on the surface of an individual cell at any one time. The differential expression of stock 51 type A and B surface antigen genes (51A and 51B) is regulated at the level of transcription. Previously, we reported that nucleotide sequences upstream of position -26 (relative to the start of translation) in the 51A and 51B surface antigen genes are necessary for transcriptional activity but are not sufficient to direct differential transcriptional control. In this report we demonstrate that at least some of the critical elements necessary for differential transcription of the 51A and 51B genes lie within the 5' coding region. A hybrid gene that contains 51B upstream sequences (-475 to +1) attached to the ATG start codon of 51A is not cotranscribed with the 51B gene. In contrast, further substitution with 51B sequences (-1647 to +885) allows the chimeric gene to be coexpressed with 51B. A different hybrid gene containing a substitution of 51B sequence from -26 to +885 in the 51A gene is also coexpressed with 51B, revealing that the critical elements within the coding region of 51B do not require 51B upstream sequences for their effect. Coinjection of the 51A gene with the chimeric gene that contains 51B up to +885 showed that the same sequences that allow coexpression with 51B prevent cotranscription with 51A. Together, these results demonstrate that a region downstream of the transcriptional start site between nucleotide positions +1 and +885 (relative to translational start) is necessary to control differential transcriptional activity.
机译：四脲草履虫原种51可以表达至少11种不同类型的表面抗原，但在任何一次仅在单个细胞的表面上表达一种。库存51型A和B型表面抗原基因（51A和51B）的差异表达在转录水平受到调控。以前，我们报道了51A和51B表面抗原基因中位置-26（相对于翻译开始）上游的核苷酸序列对于转录活性是必需的，但不足以指导差异转录控制。在本报告中，我们证明了51A和51B基因差异转录所必需的至少一些关键元件位于5'编码区内。包含与51A的ATG起始密码子相连的51B上游序列（-475至+1）的杂合基因不会与51B基因共转录。相反，进一步用51B序列取代（-1647至+885）可使嵌合基因与51B共表达。在51A基因中包含一个从-26到+885的51B序列取代的不同杂种基因也与51B共表达，这表明51B编码区内的关键元件不需要51B上游序列即可发挥作用。将51A基因与包含51B直至+885的嵌合基因共注射显示，允许与51B共表达的相同序列可防止与51A共转录。总之，这些结果表明，在核苷酸位置+1和+885之间（相对于翻译起始）而言，转录起始位点下游的区域对于控制差异转录活性是必需的。

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    Leeck, C L; Forney, J D;

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  • 年度 1996
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